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Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation
Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation
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Date
2013
Authors
Santiani Acosta A.
Evangelista Vargas S.
Valdivia Cuya M.
Risopatrón González J.
Sánchez Gutiérrez R.
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Volume Title
Publisher
Elsevier
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Abstract
The main objective was to study the effects, on sperm function, of the addition of two superoxide dismutase (SOD) analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation. Twelve alpaca semen samples were collected using an artificial vagina and then diluted at a 1:3 ratio in an extender based on skim milk, egg yolk, and fructose.
Description
This research was supported by the National Science and Technology Council (CONCYTEC) of Peru, Research Superior Council of San Marcos University (CSI-UNMSM), and Center of Biotechnology in Reproduction (CEBIOR) of La Frontera University. The authors thank Juan Olazábal and other professionals and students of IVITA-Maranganí for assistance during collection of alpaca semen samples.
Keywords
tempol,
amine oxide,
antioxidant,
superoxide dismutase,
TEMPO,
acrosome,
animal,
animal disease,
article,
Artiodactyla,
cryopreservation,
DNA fragmentation,
drug effect,
male,
methodology,
sperm,
sperm preservation